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Yıl:2019; Cilt: 6; Sayı: 2>> Özet
TAM METİN
The Journal of Neurobehavioral Sciences; 2019;6(2):129-132
Türk atlet topluluğunda monokarboksil transferaz 1 (rs1049434) polimorfizmi’nin heterozigot genotipi yaygındır
MK Zelka, ES Kaşıkçı, CS Doğan, S Kapıcı, K Ulucan, M Konuk
Üsküdar Üniversitesi, İstanbul
Egzersiz, motivasyon, azim, beslenme, sahip olunan tüm genetik geçmiş gibi atletlerin başarılarına etki eden çok sayıda parametre bulunmaktadır. Optimal bir yaklaşıma sahip olmak için, bu koşulların tamamı birbiri ile tamamlayıcı olmalıdır. Bu çalışmada uzun ve kısa mesafe koşucu atletlerde laktat düzeylerini saptamada önemli olan monokarboksil transferaz 1 (MCT1) rs 1049434 polimorfizmini karşılaştırmak ve analiz etmeyi amaçladık. Çalışmamızda 15 güçlü, 15 dayanıklı toplam 30 atlet kaydedildi. DNA izolasyonları ticari olarak mevcut DNA izolasyon kiti ve genotip süreçleri real time PCR kullanılarak gerçekleştirildi. 15 güçlü atlette sırasıyla 14 (% 93) AT, 7 (%1)‘inde TT genotipi olduğu tespit edildi. Güçlü atletlerde AA genotipi tespit edilmedi. Dayanıklı toplulukta AA, AT ve TT genotipleri sırasıyla 4 (% 27), 8 (% 53) ve 3 (% 20)‘idi. Alleller sayıldığında, güçlü ve dayanıklı atletlerde sırasıyla A alleli 14 (% 47), ve 16 (% 53) olarak sayıldı. T alleli için güç ve dayanıklı topluluklarda sırası ile 16 (% 53) ve 14 (% 47) olarak sayıldı. Geneotip ve allel bakımından gruplar arasında istatistiksel bir anlamlılık bulunmamıştır. Çalışmamızda AT genotipi her iki grupta da daha yüksektir, oysa diğer allelller eşittir. Temel hedefimiz genetik yatkınlık dikkate alınarak kısa bir zaman periyodunda atletin maksimum başarıya ulaşmak için doğrudan mı ya da taşıyıcı mı bağlantısını kurabilmektir.
Heterozygous genotype of monocarboxyl transferase 1 (rs1049434) polymorphism commons in a turkish athlete cohort
There are many parameters that have effects the success of athletes, like exercise, motivation, determination, nutrition, all of which have genetic backgrounds. To have the optimal approach, all of these conditions should complement each other. In this study, we aimed to analyze and compare the monocarboxyl transferase 1 (MCT1) rs1049434 polymorphism, which is important to determine the lactate levels, in long and short distance running athletes. A total of 30 athletes, 15 of which were power athletes and 15 were endurance, were enrolled for the study. DNA isolations were carried out by using commercially available DNA isolation Kit, and genotyping process was by real-time PCR. Of the 15 power athletes, 14 (93%) and 1 (7%) athletes had AT and TT genotypes, respectively. We detected no AA genotype in power athletes. In endurance cohort, 4 (27%), 8 (53%) and 3 (20%) athletes had AA, AT and TT genotypes, respectively. When we count the alleles, A allele was counted as 14 (47%) and 16 (53%) in power and endurance athletes. For T allele, 16 (53%) and 14 (47%) were counted in power and endurance cohorts. No statistically significant difference was found between groups in the terms of genotype and allele. In our cohort, AT genotype was higher in both groups, whereas both alleles were equal in our cohort. Our basic goal is to be able to channel the athlete correctly and carry it to maximum success in a shorter period of time considering the genetic predisposition.

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