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Yıl:2016; Cilt: 3; Sayı: 1>> Özet
TAM METİN
The Journal of Neurobehavioral Sciences; 2016;3(1):3-6
Sıçan Beyin Dokusundaki Serotonine Yönelik Oksijenli ve Oksijensiz Çalışma Etkisi
R Flora, T Theodorus, M Zulkarnain, RA Juliansyah, S Syokumawena
Sriwijaya University, Endonezya
Fiziksel egzersiz sağlığımızı korumamızda hayati bir rol oynar. Moleküler seviyede fiziksel egzersiz serotonin gibi sinir ileticilerinin salgılanmasına neden olur. Serotonin eksikliği stres ya da depresyon durumuna sebep olabilir. Fiziksel egzersizin beyindeki serotonin seviyesini artırdığını varsaydık. Bu nedenle, bu çalışmada erkek Wistar sıçanları beyin dokusundaki serotonine yönelik oksijenli ve oksijensiz çalışma etkisini araştırmayı amaçladık. 28 adet erkek Wistar sıçanı, bir haftada gerçekleştirilen 1x, 3x, 7x’li oksijenli ve oksijensiz çalışma grupları olmak üzere 7 adet kontrol grubuna ayrılmıştır. Sıçan çarkı, oksijensiz çalışma için 20 dakika boyunca 35 m/min hızda kullanılırken oksijenli çalışma için 30 dakika boyunca 20 m/min hızda kullanılmıştır. Serotonin seviyesi ST/5-HT (Serotonin/5- Hidroksitriptamin) ELISA Kit kullanılarak ölçülmüştür. Tedaviler arasında önemli farklılıklar ANOVA (α = 5%) ile test edilmiştir. Varsayımımızın aksine, hem oksijenli ve hem oksijensiz çalışma gruplarının kontrol grubundan daha düşük bir serotonin seviyesine sahip olduğu ortaya çıktı.
Effect of Aerobic and Anaerobic Exercise toward Serotonin in Rat Brain Tissue
Physical exercise plays a substantial role in maintaining our heath. In the molecular level, physical exercise induces the release of neurotransmitter, such as serotonin. Lack of serotonin could lead to stress or depression condition. We assumed that physical exercise could increase serotonin level in the brain. Therefore, this study aimed to investigate the effect of anaerobic and aerobic exercise toward serotonin level in male Wistar rat brain tissue. Twenty-eight male Wistar rats were divided into seven groups consist of control; 1x, 3x, 7x of aerobic exercise; and 1x, 3x, 7x of anaerobic exercise which conducted in a week. A rat treadmill was used at speed 35 m/min during 20 min for anaerobic exercise, and it was used at speed 20 m/min during 30 min for aerobic exercise. Serotonin level was measured using ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit. Significant differences between treatments were tested by ANOVA (a = 5%). In contrast, both of anaerobic and aerobic exercise had lower serotonin level than the control.

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